Reporter
Part:BBa_K1943022:Design
Designed by: Shixin Lu, Shiqiang Tang Group: iGEM16_SUSTech_Shenzhen (2016-10-13)
TRPC5-7
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 930
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 167
Illegal BglII site found at 1520 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2539
Illegal BsaI.rc site found at 1627
Illegal BsaI.rc site found at 2054
Illegal SapI site found at 1722
Design Notes
This year, TRPC5 takes a quite important role in our project(see how we do the directed evolution experiment: http://2016.igem.org/Team:SUSTech_Shenzhen/Notebook/Molecular ). Then we design primers and do PCR of this TRPC5 coding sequence and ligate it to pSB1C3 backbone.
Source
Original TRPC5:
Sequence from NCBI
Synthesis by IDT and Wuxi Qinglan Biotech Co. Ltd.